1. The CTC fluorescence was polar distributed in the zygote protoplast.
合子原生质体CTC荧光呈极性分布。
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2. Temperature remarkably affected the frequency and the speed of protoplast fusion.
温度明显影响原生质体融合的频率和速度。
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3. It provides scientific basis for the breeding of protoplast fusion of killer yeast.
为嗜杀酵母原生质体融合育种提供了依据。
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4. The result showed that the protoplast of rhizopus and candida can merge each other.
实验发现根霉和酵母有获得糖化力高，又能发酵产生酒香味的融合子的可能。
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5. The fermentative characters of the brewer, s yeast protoplast fusant GR8 were studied.
研究了啤酒酵母原生质体融合株GR8的主要发酵特性。
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6. Basic work for the system establishment about protoplast culture and physiological research.
系统建立的基础性工作：原生质体培养与生理研究。
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7. This is the first report of plant regeneration from the protoplast culture of an indica rice.
这是迄今第一个由籼稻原生质体再生植株的报道。
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8. The latest developments on protoplast culture and protoplast fusion are focused in this review.
本文综述了近年来世界各国果树原生质体培养和细胞融合的研究进展。
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9. The inactivated protoplast fusion method was carried out to select new Bacillus thuringiensis strains.
利用双灭活原生质体融合技术获得了苏云金杆菌新菌株。
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10. In this paper, the strain is cell engineering bacteria, which constructed by the way of protoplast fusion.
本文研究的是一种通过原生质体融合技术构建出的细胞工程菌。
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11. The effect of protoplast regeneration and screen was enhanced when R2YE medium was replaced by bran medium.
采用麸皮再生培养基替代R2YE再生培养基，原生质体再生率、生长及筛选效果得到明显改善。
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12. Observed by microscope, the mycelium protoplast agglomerated under the treatment of antifungal active substance.
显微镜下观察发现，活性物质能够引起菌丝原生质凝集。
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13. According to the target culture, tissue culture can be divided into organ, cell suspension and protoplast culture.
据所培养的对象，组织培养可分为器官、细胞悬浮和原生质培养。
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14. Choosing the specific depressor of mercapto-iodoacetamide (IAM) to destroy the geotrichum candidium of protoplast.
选用巯基酶的专一性抑制剂—碘乙酰氨对白地霉原生质体进行灭活处理。
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15. Predifferentiation showed that ABA was effective to increase the frequency of regeneration of protoplast-derived calli.
预分化实验表明：ABA能有效地提高原生质体愈伤组织的再生能力。
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16. The isolation, culture and fusion technology of plant protoplast have been developing rapidly over the past twenty years.
对原生质体的分离、培养、融合及植株再生方面进行了简要的论述。
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17. The results of the protoplast fusion between Rhodopseudomonas spheroides and Sacchaaromyces cerevisiae were studied in this paper.
研究了球形红假单胞菌与酿酒酵母菌的原生质体融合条件，并对融合子性能进行了测定。
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18. The regeneration system of soybean is not perfect because the differentiation of callus tissue and the protoplast regeneration are difficult.
大豆因其愈伤组织难以分化、原生质体再生困难等因素，其再生体系一直不够完善。
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19. The results indicated that protoplast formation and mutagenesis are the potential methods for strain selection and improvement in edible fungi.
研究结果表明，香菇原生质体分离诱变是一种很有应用价值的食用菌菌种选育方法。
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20. The results showed that the cellobiose medium and double layer culture method were most suitable for protoplast regeneration of Ganoderma lucidum.
结果表明，纤维二糖培养基及双层培养法比较适合灵芝原生质体再生。
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21. The results indicated that herbicide resistant gene should be used as a selection marker gene in protoplast genetic transformation of B. bassiana.
结果表明，外源基因转化球孢白僵菌原生质体时，可以以抗除草剂基因为转化筛选标记。
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22. The protoplast fusion technology of edible fungi culturing difficultly was introduced mainly, and the technology of future development was discussed.
本文着重介绍了难栽培食用菌原生质体融合技术，并概括了其发展前景。
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23. Protoplast is an important system for plant genetic improvement, and the isolation of protoplast is an essential step for the protoplast manipulation.
原生质体是植物遗传改良的重要实验体系之一，而原生质体的分离是该体系的基础。
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24. The progress in protoplast and its application in fruit breeding are summarized, and the existing problems and application prospects are also discussed.
25. Protoplast fusion is a potential method, by which the target characters, especially ones controlled by multigenes or un-cloned genes, can be transferred.
原生质体融合是转移目标性状，特别是那些多基因控制或尚未克隆目标基因控制的性状的一种很有潜力的方法。
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26. Moreover, if the young leaf pieces were plasmolyzed before incubated in enzyme solution will increased the yield and activity of protoplast 3.23% and 4.2%.
在酶解之前对材料进行质壁分离处理使原生质体的产量提高了3.23％，存活率提高了4.2％。
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27. The preparation of monokaryon protoplasts and the screening of protoplast-derived monokaryon strains are important parts in mushroom research and breeding.
研究了利用珍稀食用菌茶树菇双核菌丝进行原生质体分离和再生，以及原生质体单核菌株的筛选过程。
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28. Protoplast transformation and homologous chromosomal integration of s erythraea was an important path in changing gene involved in erythromycin biosynthesis.
糖多孢红霉菌的原生质体转化和染色体同源整合，是红霉素生物合成基因改造的重要途径。
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29. Protoplast transformation and homologous chromosomal integration of s erythraea was an important path in changing gene involved in erythromycin biosynthesis.
