1. The sensitivity of soybean cultivars to Agrobacterium tumefaciens is commonly low.
大豆品种对根癌土壤杆菌的敏感性通常较低。

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2. The plasmid was then transferred into Agrobacterium strain LBA4404.
然后将此质粒转化入农杆菌lba4404菌株。

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3. Agrobacterium transformation system is an excellent transformation system.
农杆菌转化系统是一种优良的转化系统。

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4. Fruit cell transformation of strawberry was carried out with injection of Agrobacterium.
利用农杆菌注射法对草莓果实细胞进行了转化研究。

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5. The common method of Genetic Transformation of Brassica campestris L. is Agrobacterium-mediated method.
白菜遗传转化技术中最常用的方法是农杆菌介导法。

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6. In process of transformation, pre-cultured explants and Agrobacterium-infected explants were also treated withy-ray.
在转化时,分别对在感染前和感染后对外植体进行了辐射处理。

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7. The new constructed vector, 1305-3, was transformed to the two agrobacterium strains, LBA4404 and EHA105, respectively.
经大肠杆菌转化后对转化子进行酶切验证,并将新构建的载体1305-3分别转入农杆菌菌株LBA4404和EHA105。

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8. This paper investigated inhibition of Agrobacterium rhizogenes by tea catechin and screening of the anti-polyphenol strain.
本文研究了茶叶儿茶素对发根农杆菌的抑制作用并进行了抗酚菌株的筛选。

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9. Agrobacterium and gene gun have aroused most attention among various genetic transformation methods for exogenous gene into maize.
目前外源基因导入玉米受体细胞的方法很多,最受瞩目的是农杆菌介导法和基因枪法。

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10. The agrobacterium of the invention has not only heterotrophic nitrification capability but also aerobic denitrification capability.
本发明的土壤杆菌不但具有异养硝化的能力,同时具有好氧反硝化的能力。

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11. The proper negative pressure, when agrobacterium and callus cultivated, could increase transformable frequency and survival rate of callus.
农杆菌与愈伤组织混合培养时给予适当的负压条件可提高愈伤组织转化频率和存活率。

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12. The present invention improves the callus of inducing and the bud inducing of the explants, and enhances the agrobacterium-mediated ability.
本发明方法改良了外植体愈伤和生芽的诱导,提高了农杆菌介导的能力。

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13. Therefore, carbenicillin was the preferable choice of the two tested antibiotics for Agrobacterium tumefaciens-mediated transformation of tall fescue.
因此,在农杆菌介导高羊茅遗传转化中,抑菌剂以选用羧苄青霉素较为合适。

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14. The Agrobacterium-mediated transient expression analysis in intact tissues has emerged as a rapid and useful method to study gene silencing in plants.
农杆菌介导的瞬时表达体系是一个研究整株植物基因沉默的快速实用系统。

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15. Applying to leaflet regeneration system of peanut, genetic transformation of peanut using Agrobacterium-mediated approach were carrying on the research.
应用花生胚小叶再生体系,以农杆菌介导法对质粒上的基因转化进行初步探讨。

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16. Methods Hairy root of H. niger was obtained from cotyledon explants after infected with Agrobacterium rhizogenes strains A4, LBA9402, and C58C1, respectively.
方法分别用A4、LBA9402和C58C1三种发根农杆菌菌株转化莨菪子叶受体,获得发根;

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17. The MI15 strain of Agrobacterium radiobacter can produce Agrocin MI15 which inhibits the growth of Agrobactium tumefaciens, a pathogen causing grape crown gall.
在平皿培养时,能够产生农杆菌素MI15,能抑制葡萄根癌土壤杆菌的生长。

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18. The pea lectin gene was transformed into the haploid tobacco via Agrobacterium mediation using leaf disc method and the regenerated tobacco plants were obtained.
通过农杆菌介导,采用叶盘法将豌豆凝集素基因转化单倍体烟草,获得了转基因再生植株。

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19. The HAL1 gene was transformed into tobacco by Agrobacterium through leaf disc transformation and the salt tolerance character of transgenic tobacco was analysised.
用农杆菌介导的叶盘法将HAL1基因转入模式植物烟草,对转基因烟草的耐盐性进行评价;

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20. The review introduced the theory and methods of Agrobacterium-mediated transformation of peanut, and the factors influencing transformation efficiency were discussed.
介绍农杆菌介导的花生遗传转化的原理和方法并对影响转化率的因素进行了探讨。

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21. For effective elimination of Agrobacterium and screen of transformed cells after co-cultivation, sensitivity of cabbage cultured in vitro to 5 antibiotics was testes.
为了能有效地杀除共培养后残余的农杆菌和筛选转化体,测定了青菜对5种抗生素的敏感性。

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22. At present, Agrobacterium mediated transformation of leaves are widely adopted in apples, and transgenic shoots were mainly obtained from adventitious bud regeneration.
目前,农杆菌介导法是苹果上应用的主要转化方法,叶片再生不定芽途径是被广泛应用的受体系统。

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23. It was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into Zhonghua 11, a rice variety, by Agrobacterium meditation.
将其与GUS报告基因融合在一起,构建了植物表达载体,并由农杆菌介导法导入水稻品种‘中花11’中。

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24. In this study, a high efficient Agrobacterium-mediated transformation system has been established and a large-scale rice enhancer trapping population has been generated.
建立水稻突变体库是功能基因组学研究的有效手段,而高效的水稻转化体系是建立突变体库的重要基础。

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25. In this paper, the effects of milling, disruption with organic solvent and ultrasonic on disruption of Agrobacterium tumefaciens KY-3085(ATCC 4452) Strain were compared.
考察了研磨法、有机溶剂法和超声波破碎法对根瘤土壤杆菌细胞破碎的影响。

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26. Using Agrobacterium vacuum infiltration method the plant bivalent expression vector pBI121 containing GUS as a report gene was transformed into lettuce Lactuca sativa l.
以GUS基因作为报告基因,利用农杆菌真空渗透法,采取正交试验设计对莴苣LactucasativaL。

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27. The tomato iron transporter gene (LeIRT2) was introduced to Malus robusta Rehd. via Agrobacterium-mediated transformation to produce iron-deficiency tolerant apple rootstock.
用农杆菌介导法,成功地将番茄铁转运蛋白基因导入了苹果砧木八棱海棠。

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28. Achyranthes bidentata BL, a famous medica plant, was transferred by ri plasmid with Agrobacterium rhizogenes, and hairy root with kanamycin resistance was induced successfully.
用发根农杆菌(agrobacteriumrhizogenes)的ri质粒转化药用植物牛膝(achyranthes bidentataBl。),诱导出了抗卡那霉素的发状根。

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29. Achyranthes bidentata BL, a famous medica plant, was transferred by ri plasmid with Agrobacterium rhizogenes, and hairy root with kanamycin resistance was induced successfully.
用发根农杆菌(agrobacteriumrhizogenes)的ri质粒转化药用植物牛膝(achyranthes bidentataBl。),诱导出了抗卡那霉素的发状根。

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