1. Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
方法将特异性引物从S8546病毒感染细胞PCR扩增的产物克隆于T载体,正确的克隆纯化后测序,应用DNASTAR软件比较分析。

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2. Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
方法设计出针对各片段的特异性引物,用P CR方法从Z 37病毒感染的细胞提取细胞总rna,逆转录扩增、产物克隆t载体,纯化后测序,测定的序列应用DNASTAR软件比较分析。

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3. Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.
方法从Z 10株病毒感染的细胞提取总rna,将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定,应用dnastar软件分析比较。

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4. Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法从Z 10病毒感染的细胞提取总rna,将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定,应用dnas TAR软件分析比较。

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5. Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法从Z 10病毒感染的细胞提取总rna,将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定,应用dnas TAR软件分析比较。

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