Objective To construct a bait plasmid in bacterial two-hybrid system.
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2. 目的：构建含16个氨基酸的酵母双杂交随机环肽库。
AIM: To construct a cycle peptide library composed of 16 random amino acids with yeast two hybrid system.
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3. 结果：在同一细胞内可见桔黄色和蓝紫色颗粒双杂交信号。
Results: the double signals in orange yellow mixed with bluish violet colors were found in the same cells.
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4. 目的构建DLX4酵母双杂交系统并鉴定其自激活作用。
Objective To construct a yeast-two hybrid system of DLX4 and identify its self-activation.
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5. 所有的梨亚科是由两个远缘祖先类型双杂交演化的异源多倍体。
All the Pomoideae were allopolyploids drived from a doubled hybrid between two remote ancestral types.
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6. 细菌双杂交系统是新近建立的一种研究蛋白质间相互作用的方法。
Bacterial two-hybrid system is a newly developed method for studying protein-protein interaction.
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7. 目的：检测抗增殖蛋白在酵母双杂交系统中是否具有转录自激活作用。
Objective: To assay the transcriptional activation effect of prohibitin in yeast two hybrid system.
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8. 为进一步利用酵母双杂交技术研究与FOXL2相互作用的蛋白打下坚实基础。
These lay a rigid basis for investigating the proteins interacting with FOXL2 by yeast two-hybrid technique.
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9. 结论应用酵母双杂交系统，获得了一些候选的NECL1胞内区相互作用蛋白。
Conclusion Using yeast two-hybrid system, we got some alternative proteins which may interact with the cytoplasmic tail of NECL1.
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10. 为后续利用酵母双杂交技术筛选出ALP1的互作蛋白及构建互作图谱奠定了基础。
This will be used to screen for the interacting protein of ALP1 and construct the interaction map in the future.
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11. 采用酵母双杂交系统，我们用LNX蛋白中的PD Z结构域为诱饵，筛选相互作用蛋白。
Using the PDZ domain in LNX protein as bait, we screened for LNX-interacting proteins by yeast-2-hybrid system.
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12. 我们首先应用缺失突变和酵母双杂交试验将NEMO的二聚化域确定于251-419残基。
The dimerization domain of NEMO was mapped to residues 251-419 by deletion and yeast two-hybrid analyses.
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13. 为进一步研究这两个基因的功能及下游信号传导机制，本试验对这两个基因作了酵母双杂交分析。
In order to study the functions and downstream signal transduction mechanisms of these two genes, yeast two-hybrid analysis of the two genes was made in this paper.
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14. 在试验过程中用到了很多高级的生物学技术，比如说PCR,RT - PCR和酵母双杂交技术。
During the procedure, different molecular biology techniques were involved such as PCR RT-PCR and Yeast-two-hybrid system.
Nevertheless, huge protein network is larger than that we predict and single yeast two-hybrid system cannot solve all the problems, which need be complemented by other wags.
CONCLUSION: Yeast two-hybrid GAL4 system 3 can be utilized to fish HCV core region-interacting protein. HCV core protein does not have self-activating function.
RESULTS: human NS5ABP37 was screened and cloned from human liver cDNA library by yeast-two hybrid system 3. The murine NS5ABP37 was deduced by bioinformatics methods.
The yeast two-hybrid analysis demonstrated that CK1A and CRY2 can interact in vivo under blue light, which indicates that CK1A may play an important role in blue light signal induction of Arabidopsis.
The endocrine disrupting functions of 4 natural and synthetical estrogens, 4 phytoestrogens, 14 phenols widely used in the tap water supply system were detected using yeast two-hybrid technique.
Some new type of two-hybrid systems, such as split-ubiquitin system, protein-fragment complementation assay, repressor reconstitution assay and SOS recruitment system, have been developed recently.
Some new type of two-hybrid systems, such as split-ubiquitin system, protein-fragment complementation assay, repressor reconstitution assay and SOS recruitment system, have been developed recently.
